mRNA Extraction and qRT-PCR Analysis

Total mRNA was isolated utilizing the Trizol reagent (Invitrogen) and used for reverse transcription (Thermo Fisher Scientific). Then qRT-PCR was performed using the ABI7500 (Applied Biosystems) by mixing cDNA, primers and SYBR^®Green Real-Time PCR Master Mixes (Invitrogen). The primer sequences are included in Supplementary Material37 (link).

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Li J., He Y., Tan Z., Lu J., Li L., Song X., Shi F., Xie L., You S., Luo X., Li N., Li Y., Liu X., Tang M., Weng X., Yi W., Fan J., Zhou J., Qiang G., Qiu S., Wu W., Bode A.M, & Cao Y. (2018). Wild-type IDH2 promotes the Warburg effect and tumor growth through HIF1α in lung cancer. Theranostics, 8(15), 4050-4061.

Publication 2018

Trizol reagent ABI7500 SYBR®Green Real-Time PCR Master Mixes

Cdna Mrna Primer Real time pcr Reverse transcription Sybr green Trizol

Corresponding Organization :

Other organizations : Xiangya Hospital Central South University, Central South University, Kunming Medical University, Fudan University, Zhongshan Hospital, University of Minnesota, Hormel (United States), National Engineering Research Center of Human Stem Cells

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Variable analysis

independent variables

Experimental conditions that were manipulated by the researchers, but not explicitly mentioned in the provided input

dependent variables

Gene expression levels measured by qRT-PCR

control variables

Experimental conditions that were kept constant to ensure valid results, but not explicitly mentioned in the provided input

controls

Positive controls: Not specified
Negative controls: Not specified

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