Measurement of Maternal and Fetal IgG Subclasses
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : University of Hawaiʻi at Mānoa
Other organizations : Université de Yaoundé I, Case Western Reserve University, Center for Global Health
Protocol cited in 1 other protocol
Variable analysis
- Immunoglobulin G IgG1 and IgG3 subclasses
- Median fluorescence intensity
- Plasma samples dilution (1:100)
- Incubation time (1 hour)
- Incubation temperature (25°C)
- Concentrations of antibodies (mouse anti-human IgG1 at 1:250 dilution, mouse anti-human IgG3 at 1:2000 dilution, phycoerythrin-labeled donkey anti-mouse IgG H + L at 1:250 dilution)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!