Immunofluorescent Microscopy for Cell Quantification

Sections that were stained with immunobiological stains and immunofluorescent stains were digitized to capture a minimum of 1936 × 1460 pixels covering one entire cross-section utilizing an M2 Microscope (Carl Zeiss) with an Axiocam 503 color camera (Carl Zeiss). These slides were analyzed using ZEN 2 blue edition version 2.0 (Carl Zeiss) as described elsewhere^{4 (link),11 (link),16 (link),30 (link)}. Staining index was obtained by number of positive cells/ total number of cells × 100.

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Cai C., Kilari S., Zhao C., Singh A.K., Simeon M.L., Misra A., Li Y., Takahashi E., Kumar R, & Misra S. (2021). Adventitial delivery of nanoparticles encapsulated with 1α, 25-dihydroxyvitamin D3 attenuates restenosis in a murine angioplasty model. Scientific Reports, 11, 4772.

Publication 2021

M2 Microscope Axiocam 503 color ZEN 2 blue edition version 2.0

Cells Immunofluorescent Microscope Stains

Corresponding Organization :

Other organizations : Union Hospital, Huazhong University of Science and Technology, Mayo Clinic, Mayo Clinic in Arizona

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Variable analysis

independent variables

Immunobiological stains
Immunofluorescent stains

dependent variables

Staining index (number of positive cells / total number of cells × 100)

control variables

The entire cross-section was digitized to capture a minimum of 1936 × 1460 pixels
Microscope used: M2 Microscope (Carl Zeiss)
Camera used: Axiocam 503 color camera (Carl Zeiss)
Software used: ZEN 2 blue edition version 2.0 (Carl Zeiss)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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