Polyphenolic Compound Identification Using QTOF

Polyphenolic compounds were identified by using an Agilent 6520 Accurate-Mass quadrupole time of flight (QTOF) machine described by Suleria et al. [18 (link)] after modifications. Column, gradient, chromatographic, and other machine conditions were the same as we reported [11 (link),19 (link)]. MassHunter Workstation Software (version B.06.00) (Agilent, Santa Clara, CA, USA) was used to extract and identify phytochemicals. The mass spectra of twenty-four external standards were also obtained and standard equations were generated as described by Ali et al. [20 (link)].

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Ali A., Kiloni S.M., Cáceres-Vélez P.R., Jusuf P.R., Cottrell J.J, & Dunshea F.R. (2022). Phytochemicals, Antioxidant Activities, and Toxicological Screening of Native Australian Fruits Using Zebrafish Embryonic Model. Foods, 11(24), 4038.

Publication 2022

MassHunter Workstation Software

Chromatographic Mass spectra Phytochemicals

Corresponding Organization : University of Leeds

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Variable analysis

independent variables

Modifications to the Agilent 6520 Accurate-Mass quadrupole time of flight (QTOF) machine

dependent variables

Identification of polyphenolic compounds

control variables

Column
Gradient
Chromatographic conditions
Other machine conditions

controls

Twenty-four external standards were obtained, and standard equations were generated

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