The assays were carried out as described previously (31 (link)). Culture samples were diluted 1:1000 in 1 ml of PBS. Fluorescence intensity of mNeonGreen and mCherry in cells was monitored by flow cytometry analysis on a BD FACSJazz (BD Biosciences), and data were acquired with the BD FACS Sortware. mNeonGreen and mCherry were excited with 488 and 561 nm solid-state lasers, and their emission was detected using 513/17 and 610/20 nm emission filters, respectively. For each sample, fluorescence of 20 000 cells was captured, and the data was analysed using FCS Express 7 (De Novo Software).
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Flow Cytometric Analysis of Fluorescent Proteins
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Corresponding Organization : Université de Sherbrooke
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Variable analysis
independent variables
- Dilution of culture samples (1:1000 in 1 ml of PBS)
- Fluorescence intensity of mNeonGreen
- Fluorescence intensity of mCherry
- Excitation wavelengths (488 nm for mNeonGreen, 561 nm for mCherry)
- Emission filters (513/17 nm for mNeonGreen, 610/20 nm for mCherry)
- Flow cytometry analysis on a BD FACSJazz
- Data acquisition with BD FACS Sortware
- Analysis using FCS Express 7 (De Novo Software)
- Positive control: Not specified
- Negative control: Not specified
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