Bacterial Adhesion on Apatite Pellets

Every experiment was conducted with the first step of cultivating the relevant bacterial strains overnight. The reproducible results were ensured using the same optical density (OD) of 1. Of note, even with the same OD, the absolute cell number somewhat differs within the strains due to the cell size and OD is not strictly linearly related to the cell count except within a limited range. Thus, the more reliable way to obtain reproducible conditions is by using cfu counting. Yet, precedent experiments of the correlation of OD and cfu showed that, despite the differences in size and shape of the bacterial strains, the number of cfu are within the same range. This bacterial solution was applied on the apatite pellets placed in an MTP. An incubation period of 24 h at 37 °C followed. The experiments were performed in triplicate (n = 3) and the results are provided in terms of mean ± standard deviation.