Sample separation (5 μL) was performed on 5 cm × 10 cm silica gel 60 plates (Merck, Darmstadt, Germany). Plates were first developed to a distance of 90 mm with ethyl acetate/glacial acetic acid/water (2:2:1, v/v/v) as mobile phase at room temperature. After being dried in air, the plates were redeveloped to a distance of 95 mm with the same mobile phase. Sugars were colorized with aniline-diphenylamine-phosphoric acid solution by heating at 105 °C for 10 min, and photographed.
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