Gastric Cancer Cell Line Characterization

HGC-27 and AGS gastric cancer cells were purchased from Shanghai Cell Bank of Chinese Academy of Sciences, MKN45 and GES-1 were gifts from Digestive Tumor Research Institute of Fujian Medical University. HGC-27, GES-1 and MKN45 cells were cultured in RPMI 1640 medium with 10% fetal bovine serum (Cat.11875–093, Cat.10099–141, Gibco,Thermo Fisher Scientific, Shanghai, China) and 1% Penicillin/Streptomycin Solution (100×, Gibco, Thermo Fisher Scientific, Shanghai, China). The total protein lysates were blotted with following antibodies: mouse anti-Pin1 (1:3000) from professor Lu of Harvard Medical University^{27 (link)}. Mouse anti-Actin (1:3000,#HC201–02; TransGen Biotech, Beijing, China).The following antibodies were purchased from Cell Signaling Technology: rabbit anti-Cyclin D1 (#2978,1:1000), rabbit anti-Phospho-Akt(Ser473) (#9271, 1:1000); rabbit anti-β-Catenin(#8480, 1:1000); rabbit anti-Phospho-GSK3beta(Ser9)(#9336,1:1000); rabbit anti-c-Myc (#9402, 1:1000), rabbit anti-CyclinD1(#2922, 1:1000), rabbit anti-CyclinE(#20808, 1:1000). All-trans retinoic acid (ATRA) powder were purchased from Sigma, ATRA-releasing pellets were from Innovative Research of America. SPF BALB/c nude mice were raised in Laboratory Animal Center of Fujian Medical University. All of animal experiments were approved by Experimental Animal Ethics of Fujian Medical University.