Lenses were dissected from P2 control and Dlgf/f10Cre mice, and cytosolic- and cytoskeletal-associated protein lysates were prepared by Triton X-100 extraction, as previously described.10 (link) The lysates (50 μg each) were electrophoresed, the proteins were transferred to polyvinylidene difluoride (PVDF) membranes, and the membranes were blotted for with mouse anti–N-cadherin (BD Biosciences Cat# 610921), rabbit anti-human β-catenin (Sigma-Aldrich Corp., St. Louis, MO, USA, Cat# C2206), rabbit anti–human-active β-catenin (Millipore, Billerica, MA, USA, Cat# 05-665), or goat anti-mouse EphA2 (R&D Systems, Minneapolis, MN, USA, Cat# AF639) antibodies at a 1:100 dilution. The blots were reprobed with mouse anti-rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Millipore Cat# MAB374) as a loading control. Bands were visualized using the Enhanced Chemiluminescence Plus kit (ECL plus, ThermoScientific, Rockford, IL, USA), and protein levels were quantified by phosphorimager analysis on a Storm Scanner. At least three pools were generated, and each pool was analyzed in triplicate over one to three blots. Relative protein levels were calculated by setting the protein/Gapdh ratio for the controls at 1.0. The data reported are the mean ± SD across three to four pools.