DOX-loaded SF7761 GMs-derived exosomes were labelled via using Exo-green (Cat# EXOG200A-1, System Biosciences) as per the manufacturer’s instructions. Equal amounts of labelled SF7761 exosomes were incubated with SF7761- or U251- GMs for 4–24 hours, and they were counterstained with phalloidin-rhodamine, a red fluorescent marker for labeling the membrane of cells, and DAPI, a blue fluorescent marker for labeling nuclei of cells. The uptake of DOX-loaded SF7761 GMs-exosomes (Exo-Dox) was imaged with SF7761- and U251- GMs using a confocal microscope (Zeiss Laser Scanning Microscope LSM 880 NLO with Airyscan) at 60x objective setup, as described previously.37 (link),38 (link)
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Exosome-mediated Doxorubicin Delivery to Glioma Cells
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Cat 1
Confocal microscope
Dapi
Exosomes
Laser scanning microscope
Nuclei of cells
Rhodamine phalloidin
Corresponding Organization : City University of Hong Kong
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Variable analysis
independent variables
- Incubation of labelled SF7761 exosomes with SF7761- or U251- GMs for 4–24 hours
- Uptake of DOX-loaded SF7761 GMs-exosomes (Exo-Dox) by SF7761- and U251- GMs
- Exo-green labeling of DOX-loaded SF7761 exosomes
- Counterstaining with phalloidin-rhodamine (red fluorescent marker for cell membrane) and DAPI (blue fluorescent marker for cell nuclei)
- No positive or negative controls were explicitly mentioned in the provided information.
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