Establishment and Characterization of Anaplastic Thyroid Cancer Cell Lines

Anaplastic thyroid cancer cell lines (SW1736, 8505C, OCUT-2, and KTC-2) were cultured in RPMI 1640 growth media with L-glutamine (300 mg/L), sodium pyruvate and nonessential amino acids (1%) (Corning Inc, Corning, NY, USA), supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) and penicillin-streptomycin (200 IU/L) (Corning) at 37°C, 5% CO₂, and 100% humidity. Lentivirally modified SW1736 cells were generated as recently described (11 ) with either an empty vector (SW-EV) or to overexpress TRβ (SW-TRβ). SW-EV and SW-TRβ were grown in the above conditions with the addition of 1 μg/ml puromycin (Gold Bio, St Louis, MO, USA). SW1736 and KTC-2 were authenticated by the Vermont Integrative Genomics Resource at the University of Vermont using short tandem repeat profiles and Promega GenePrint10 System (SW1736, May 2019; KTC-2, October 2019). 8505C and OCUT-2 were authenticated by University of Colorado by short tandem repeat profiles (8505C, June 2013; OCUT-2, June 2018). TheSW1736 and KTC-2 cells were tested for mycoplasma by PCR as described by Uphoff et al (SW1736, April 2019; KTC-2, February 2020; 85050C, February 2020; OCUt-2, January 2020) (13 (link)).