The sensitivity of BC cells to OX was measured using a CCK-8 assay kit (Dojindo Molecular Technologies, Inc.). In short, BT474/OX or MCF-7/OX cells (5 × 104 cells/well) were seeded into 96-well plates and exposed to OX in a concentration gradient from 0 to 20 μmol/L for 48 h. Then, the cells were cultured for another 2 hours after the 10 μL CCK-8 reagent (Beyotime, China) was added to the plates. The absorbance was detected using a microplate reader (BioTek China) at 450 nm.
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Assessing Breast Cancer Cell Sensitivity to OX
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Corresponding Organization : Nanjing Medical University
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Variable analysis
independent variables
- Concentration of OX
- Cell viability
- Cell line (BT474/OX or MCF-7/OX)
- Seeding density (5 × 10^4 cells/well)
- Incubation time (48 h for OX treatment, 2 h for CCK-8 reagent)
- Measurement method (CCK-8 assay, absorbance at 450 nm)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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