Immunoaffinity Purification and Mass Spectrometry
Corresponding Organization :
Other organizations : Howard Hughes Medical Institute, Johns Hopkins University
Protocol cited in 4 other protocols
Variable analysis
- Substrates from in vitro proteolysis assays or transformed/transfected cells (lysed in RIPA buffer)
- Proteins analyzed by MALDI-TOF mass spectrometry
- Anti-Flag immunoaffinity purification with the M2 resin (Sigma, St Louis, MO)
- Use of sinapinic acid as the matrix for MALDI-TOF mass spectrometry
- Positive control: Not specified
- Negative control: Not specified
Annotations
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