Total RNA was extracted from lung tissue at Rosetta Inpharmatics Gene Expression Laboratory (Seattle, WA, USA). mRNA profiling was performed using a custom-made Affymetrix HU133 array (GPL 10379) containing 751 controls probe sets and 51,627 non-control probe sets. Gene expression was normalized with the Robust Multichip Average method implemented in the Affymetrix Power Tools software [20 (link)].
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