Bioluminescent Imaging of Circadian Rhythms

Brains were removed and sectioned as reported previously (Maywood et al., 2006 (link)). Bioluminescent emissions from PER2::LUC SCN and lung slices were recorded using photomultiplier tubes (PMT; Hamamatsu) and CCD cameras (Hamamatsu) as described previously (Maywood et al., 2013 (link)). Lentiviral transduction of SCN slices was performed as described previously (see Supplemental Experimental Procedures) (Brancaccio et al., 2013 (link)).

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Parsons M.J., Brancaccio M., Sethi S., Maywood E.S., Satija R., Edwards J.K., Jagannath A., Couch Y., Finelli M.J., Smyllie N.J., Esapa C., Butler R., Barnard A.R., Chesham J.E., Saito S., Joynson G., Wells S., Foster R.G., Oliver P.L., Simon M.M., Mallon A.M., Hastings M.H, & Nolan P.M. (2015). The Regulatory Factor ZFHX3 Modifies Circadian Function in SCN via an AT Motif-Driven Axis. Cell, 162(3), 607-621.

Publication 2015

CCD cameras

Brains Lung

Corresponding Organization : Mary Lyon Centre at MRC Harwell

Other organizations : MRC Laboratory of Molecular Biology, New York University, New York Genome Center, University of Oxford, Genomics (United Kingdom), University of Tsukuba, Erasmus MC

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Variable analysis

independent variables

Lentiviral transduction of SCN slices

dependent variables

Bioluminescent emissions from PER2::LUC SCN and lung slices

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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