Modulation of BLV LTR Promoter

CC81LTRBLVGFP cells were incubated for 24 hours to form a confluent monolayer.
Subsequently, the cells were treated with 10mM VPA or 500nM TSA for 24 hours. To evaluate the effect of INF-α on BLV LTR promoter, CC81LTRBLVGFP cells were treated without or with 75, 125, 250, 500 or 1000 U/mL of INF-α for 48 hours. The percentage of GFP positive cells was determined by flow cytometry as described below.

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Rammauro F., Fló M., Carrión F., Ortega C., Nunzio F.D., Vallmitjana A., Pritsch O., & Olivero‐Deibe N. (2024). A versatile reporter system to study cell-to-cell and cell-free bovine leukemia virus infection.

Publication 2024

Corresponding Organization : Institut Pasteur de Montevideo

Other organizations : Universidad de la República, Université Paris Cité, Institut Pasteur, Irvine University, University of California, Irvine

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Variable analysis

independent variables

Treatment with 10mM VPA
Treatment with 500nM TSA
Treatment with 75, 125, 250, 500 or 1000 U/mL of INF-α

dependent variables

Percentage of GFP positive cells

control variables

Cell line: CC81LTRBLVGFP
Incubation time: 24 hours to form a confluent monolayer
Treatment duration: 24 hours for VPA and TSA, 48 hours for INF-α

controls

Untreated CC81LTRBLVGFP cells (negative control)

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