Immunohistochemical Detection of H. pylori

H. pylori were detected in infected gastric tissues by immunohistochemistry (IHC) using a rabbit anti-H. pylori antibody (Abcam, Milton, Cambridge, UK). Positive-staining cells were visualized with diaminobenzidine (DAB) (Vectastain Elite ABC Kit for rabbit; Vector Laboratories, Burlingame, CA, USA) and morphometrically analyzed with Leica DM 2500 microscopy and Leica Application Suite software (version 4.4; Leica microsystems, Heerbrugg, Switzerland). The evaluation of H. pylori-positive cells as marker of the infection was performed by counting the H. pylori-positive cells distributed in the non-infected control gastric tissue. Paraffin embedded longitudinal sections of antrum and corpus were stained with hematoxylin and eosin (H&E) and evaluated. It was graded for gastritis and mucosal changes and analyzed by a double blind test according to the grading scheme for rodents [32 (link),37 (link)].

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Woo H.J., Yang J.Y., Lee M.H., Kim H.W., Kwon H.J., Park M., Kim S.K., Park S.Y., Kim S.H, & Kim J.B. (2020). Inhibitory Effects of β-Caryophyllene on Helicobacter pylori Infection In Vitro and In Vivo. International Journal of Molecular Sciences, 21(3), 1008.

Publication 2020

rabbit anti-H. pylori antibody Vectastain Elite ABC Kit for rabbit Leica Application Suite software

Anti antibody Antrum Cells Embedded paraffin Eosin Gastric Gastritis H pylori Immunohistochemistry Infection Microscopy Mucosal Rabbit Rodents Tissue Vector

Corresponding Organization : Semyung University

Other organizations : Daegu Haany University, National Forensic Institute, Daekyeung University, Dankook University

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Variable analysis

independent variables

Infection with H. pylori

dependent variables

H. pylori-positive cells in gastric tissues
Gastritis and mucosal changes

control variables

Non-infected control gastric tissue

controls

Positive control: H. pylori-positive cells in infected gastric tissues
Negative control: H. pylori-positive cells in non-infected control gastric tissue

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