The following parameters were determined using the Western blotting assay: HMGB-1, AIF, LC3BII/I, and Beclin-1 (primary antibodies were purchased from Thermo Scientific Co., IL, USA), and the steps adhered to the formerly mentioned technique by El Gazar et al. (2022) [82 (link)]. Specific bands were visualized with a ChemiDocTM imaging system (Image LabTM software version 5.1, Bio-Rad Laboratories Inc., Hercules, CA, USA). The optical density (OD) of the following findings was standardized against β-actin.
ActinAntibodies Beclin 1 Findings Hmgb Western blotting assay
Corresponding Organization : October 6 University
Other organizations :
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université de Bordeaux, Unité de Recherche Œnologie, Institut Polytechnique de Bordeaux, National Research Centre, MetaboHUB, Cairo University, Alfaisal University, Al-Azhar University, Menoufia University, Zagazig University, University of Jordan
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