Flow Cytometry Analysis of Lung Cells

Flow cytometry was performed as described previously [47 (link), 48 (link)]. The cells were incubated CD44-APC (BD Pharmingen), however the control were incubated with IgG. After incubation, the cells were suspended in PBS for Flow Cytometry analysis. Mice were sacrificed, and lung tissues were scissors cut into a meaty shape and digested. Cells were stained with fluorescently labeled antibodies. Then cells were analyzed by FACS (BD Biosciences). Finally, the data was analyzed in the Flowjo 7.6.1software. All antibodies information was listed in Supporting Information, Table S2.