Western Blotting Antibody Panel

The following antibodies for the purpose of Western blotting were acquired from Cell Signaling Technologies: pAKT S473 (Catalog number 4060), PAN-AKT (#2920), Phospho-p44/42 MAPK (#4370), p44/42 MAPK (#4695), S6 Ribosomal Protein (#2317), Phospho-S6 Ribosomal Protein (#4858), Phospho-p70S6 Kinase Thr389 (#97596), p70S6 Kinase (#9202), Phospho-4EBP1 Thr37/46 (#2855), 4EBP1 (#9452). Antibodies against Beta-Actin were purchased from GeneTex (catalog number 109639). MAPK inhibitors were purchased from Tocris: FR180204 (catalog number 3706), U0126 (catalog number 1144), PD980595 (catalog number 1213). Sapanisertib (Cat# HY-13328) and Rapalink-1 (Cat# HY-111373) were purchased from MedChemExpress. Torin-1 (Cat #4247) was purchased from Tocris. PI3K/AKT/mTOR inhibitors BKM120 (Buparlisib; Novartis), AZD5363 (Capivasertib; Selleckchem), and RAD001 (Everolimus, MedChemExpress Cat# 159351–69–6) were generously provided by Dr Kathleen Millen.

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Wehle D.T., Bass C.S., Sulc J., Mirzaa G, & Smith S.E. (2023). Protein interaction network analysis of mTOR signaling reveals modular organization. The Journal of Biological Chemistry, 299(11), 105271.

Publication 2023

4ebp1 Antibodies Azd5363 Beta actin Buparlisib Capivasertib Everolimus Fr180204 Inhibitors Mtor P44 mapk P70s6 kinase Pi3k inhibitors bkm120 Rad001 Rapalink 1 Ribosomal protein Sapanisertib Torin U0126

Corresponding Organization :

Other organizations : University of Washington, Brotman Baty Institute

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Variable analysis

independent variables

MAPK inhibitors: FR180204, U0126, PD980595
PI3K/AKT/mTOR inhibitors: BKM120 (Buparlisib), AZD5363 (Capivasertib), RAD001 (Everolimus)
Sapanisertib
Rapalink-1
Torin-1

dependent variables

Protein expression/phosphorylation levels of: pAKT S473, PAN-AKT, Phospho-p44/42 MAPK, p44/42 MAPK, S6 Ribosomal Protein, Phospho-S6 Ribosomal Protein, Phospho-p70S6 Kinase Thr389, p70S6 Kinase, Phospho-4EBP1 Thr37/46, 4EBP1, Beta-Actin

control variables

Antibodies purchased from Cell Signaling Technologies and GeneTex for Western blotting

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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