Quiescent Cell Tracking Using Microfluidics

We utilized a CellASIC ONIX microfluidic profusion platform and accompanying microfluidic plates to track particles in quiescent cells (EMD Millipore, a division of Merck KGaA, Darmstadt, Germany). Quiescence was established by allowing cells to grow within the same SCD media for 7 days (Laporte et al., 2011 (link)). Seven-day-old media, collected from the culture by centrifuging 1mL of cells and harvesting the supernatant, was used as perfusion media in the microfluidic plate at a rate of 2 psi.

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Joyner R.P., Tang J.H., Helenius J., Dultz E., Brune C., Holt L.J., Huet S., Müller D.J, & Weis K. (2016). A glucose-starvation response regulates the diffusion of macromolecules. eLife, 5, e09376.

Publication 2016

CellASIC ONIX EMD Millipore

Cells Perfusion

Corresponding Organization :

Other organizations : Lawrence Berkeley National Laboratory, ETH Zurich, New York University, Centre National de la Recherche Scientifique, Université de Rennes, Institut de génétique et de développement de Rennes, University of California, Berkeley

Top 5 similar protocols

Variable analysis

independent variables

Microfluidic profusion platform (CellASIC ONIX)
Microfluidic plates
Perfusion media (7-day-old media from cell culture)
Perfusion rate (2 psi)

dependent variables

Particle tracking in quiescent cells

control variables

Quiescent cells (allowed to grow in SCD media for 7 days)
Cell culture media (SCD media)

controls

Positive control: Not specified
Negative control: Not specified

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