Maxillary Bone Loss Quantification

Maxillary samples were collected at 0, 1, 3, 6, and 10 days (Jiao et al., 2013 (link)) after surgery and scanned with a Scanco μCT50 scanner (Scanco Medical AG, Brutishly, Switzerland) with a resolution of 20 μm at 70 kVp and 200 μA. The 3D images from the buccal and palatal sides were constructed using Materialize Mimics v17.0 software. As for bone loss evaluation, the distance between the cemento-enamel junction (CEJ) and alveolar bone crest (ABC) was measured by the abovementioned software. Twelve sites from each maxillary molar were chosen for measurement, and averaged distances were calculated. Each measurement was repeated three times.

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Li D., Feng Y., Tang H., Huang L., Tong Z., Hu C., Chen X, & Tan J. (2020). A Simplified and Effective Method for Generation of Experimental Murine Periodontitis Model. Frontiers in Bioengineering and Biotechnology, 8, 444.

Publication 2020

Scanco μCT50 scanner

Alveolar crest Bone Bone loss Enamel Maxillary Molar Scanco Surgery

Corresponding Organization : Stomatology Hospital

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Variable analysis

independent variables

Days after surgery (0, 1, 3, 6, and 10 days)

dependent variables

Distance between the cemento-enamel junction (CEJ) and alveolar bone crest (ABC)

control variables

Maxillary molar samples
Scanco μCT50 scanner with a resolution of 20 μm at 70 kVp and 200 μA
Materialize Mimics v17.0 software for 3D image construction and measurement
Twelve sites from each maxillary molar used for measurement
Each measurement repeated three times

controls

No positive or negative controls were explicitly mentioned in the provided information.

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