Expansion of Human Bone Marrow Mesenchymal Stem Cells

Human Bone Marrow Mesenchymal Stem Cells (BM-hMSCs) were purchased from PromoCell, 69126 Heidelberg, Germany, and expanded as previously described [13 (link)]. Briefly, BM-hMSCs were expanded in the presence of a growth medium (GM), a high glucose-based Dulbecco’s modified Eagle’s medium (DMEM) with 2% L-glutamine/penicillin-streptomycin/amphotericin B solution, 1 mM sodium pyruvate, MEM Non Essential Amino Acids Solution 1X and 10% fetal bovine serum (FBS) (referred as complete medium FBS) or 5% human platelet lysate (hPL) (referred as complete medium hPL) at 37 °C and 5% CO₂ in an incubator.