Chlamydia Challenge Protocol for Mice

All animals received a subcutaneous injection of 2.5 mg of medroxyprogesterone acetate (Depo-Provera, Pfizer) one week prior to challenge. Three weeks after the final vaccination, mice were challenged intra-vaginally with 10⁷ IFU of Ct serovar D/UW3/Cx EBs as previously described [7] (link), [17] (link). Cervico-vaginal swabs were obtained on days 1, 3, 7, 14 and 22 post-challenge, and the total number of inclusions per well were enumerated in a blinded fashion using fluorescence microscopy.

Free full text: Click here

Paes W., Brown N., Brzozowski A.M., Coler R., Reed S., Carter D., Bland M., Kaye P.M, & Lacey C.J. (2016). Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice. Vaccine, 34(35), 4123-4131.

Publication 2016

Depo-Provera

Animals Depo provera Fluorescence microscopy Inclusions Medroxyprogesterone acetate Mice Subcutaneous injection Vaccination Vaginal

Corresponding Organization : University of York

Other organizations : Infectious Disease Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Subcutaneous injection of 2.5 mg of medroxyprogesterone acetate (Depo-Provera, Pfizer) one week prior to challenge
Challenge with 10^7 IFU of Ct serovar D/UW3/Cx EBs

dependent variables

Total number of inclusions per well enumerated in a blinded fashion using fluorescence microscopy
Cervico-vaginal swabs obtained on days 1, 3, 7, 14 and 22 post-challenge

control variables

Time between final vaccination and challenge (3 weeks)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!