Echocardiography and Hemodynamic Analysis of Rat Right Ventricular Function

After 28 days, rats were fasted overnight and initially anesthetized with isoflurane inhalation. Echocardiography was performed with Visual Sonics Vevo 2100 ultrasoundmachine and 12S rodent probe (GE Healthcare, CT, United States) to determine pulmonary artery acceleration time (PAAT) and tricuspid annulusplain systolic excursion (TAPSE). Data were analyzed with EchoPAC software (GE Healthcare, CT, United States). Then, haemodynamic analysis was performed as previously described (Xia et al., 2018 (link)). Briefly, anesthesia was given to rats with 20% ethylurethanm via injecting intraperitoneally (4 ml/kg). After intubation, right ventricular systolic pressure (RVSP) was recorded. To investigate right ventricular hypertrophy (RVH), the right ventricle (RV), the left ventricle plus septum (LV + S) and the body weight (BW) were weighed, and the RV/(LV + S) ratio and RV/BW ratio were determined.

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Dong L., Liu X., Wu B., Li C., Wei X., Wumaier G., Zhang X., Wang J., Xia J., Zhang Y., Yiminniyaze R., Zhu N., Li J., Zhou D., Zhang Y., Li S., Lv J, & Li S. (2022). Mxi1-0 Promotes Hypoxic Pulmonary Hypertension Via ERK/c-Myc-dependent Proliferation of Arterial Smooth Muscle Cells. Frontiers in Genetics, 13, 810157.

Publication 2022

12S rodent probe EchoPAC software

Acceleration Anesthesia Body weight Echocardiography Haemodynamic Inhalation Intubation Isoflurane Pulmonary artery Rats Right ventricle Right ventricular hypertrophy Rodent Systolic Systolic pressure Ventricle septum

Corresponding Organization : Fudan University

Other organizations : Wuxi People's Hospital

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Variable analysis

independent variables

Fasting duration (overnight)

dependent variables

Pulmonary artery acceleration time (PAAT)
Tricuspid annulus plane systolic excursion (TAPSE)
Right ventricular systolic pressure (RVSP)
Right ventricle (RV) weight
Left ventricle plus septum (LV + S) weight
Body weight (BW)
RV/(LV + S) ratio
RV/BW ratio

control variables

Anesthesia (isoflurane inhalation, 20% ethylurethanm via intraperitoneal injection)

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