Comprehensive NGS Profiling of Lung Cancer

In total, 12,533 samples were analysed using the DNA‐based NGS method. Library preparation for 68 lung cancer‐related genes, covering 0.345 Mb of the human genome, was performed using a DNA capture‐based NGS panel (Burning Rock Biotech, Guangzhou, PR China) (supplementary material, Table S1). A total of 100–200 ng of genomic DNA from FFPE tissues was fragmented using Covaris M220 ultrasonicator (Covaris Woburn, MA, USA). An enriched library was then used to hybridise with the capture probes. The amount and size of the library were assessed using an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Sequencing was performed using a NextSeq 550 sequencer (Illumina, Inc., San Diego, CA, USA). NGS sequence reads were aligned to the hg19 version of the human genome using BWA‐MEM 0.7.10. The data processing methods employed here have been described previously [11 (link)].