Synthesis and Purification of RNA Oligonucleotides

The RNA oligonucleotides (5′-UCAGΨCAGU-3′, 5′-ACUGACUGA-3′, 5′-UCACΨGAGU-3′, 5′-ACUCAGUGA-3′) were synthesized on an Applied Biosystems DNA/RNA synthesizer, deprotected and purified according to previously published procedures^{31 (link),53 (link),54 (link)}. For NMR study, each of the obtained duplexes was dissolved in a volume of 200 μl of 90% H₂O and 10% D₂O solution and placed into a 3 mm NMR sample tube (Supplementary Methods, Section 1).

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Deb I., Popenda Ł., Sarzyńska J., Małgowska M., Lahiri A., Gdaniec Z, & Kierzek R. (2019). Computational and NMR studies of RNA duplexes with an internal pseudouridine-adenosine base pair. Scientific Reports, 9, 16278.

Publication 2019

DNA/RNA synthesizer

Oligonucleotides

Corresponding Organization :

Other organizations : Institute of Bioorganic Chemistry, Polish Academy of Sciences, Adam Mickiewicz University in Poznań, University of Calcutta

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Variable analysis

independent variables

Synthesized RNA oligonucleotides (5′-UCAGΨCAGU-3′, 5′-ACUGACUGA-3′, 5′-UCACΨGAGU-3′, 5′-ACUCAGUGA-3′)

dependent variables

NMR study of each of the obtained duplexes

control variables

Dissolved in a volume of 200 μl of 90% H2O and 10% D2O solution
Placed into a 3 mm NMR sample tube

controls

No positive or negative controls were explicitly mentioned in the provided information.

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