Multiscale Retinal Electrophysiology

Mice were dark-adapted overnight by placing their home cage in a light-shielded box fitted with an air pump for circulation. All dissection procedures the day of the experiment were carried out in complete darkness using infrared converters and cameras. Mice were decapitated, eyes enucleated, and placed into oxygenated room temperature Ames solution (Sigma, A1420) during retinal dissection and vitrectomy as described previously (Yao et al., 2018 (link)). An ~1 × 2 mm piece from dorsal retina was placed RGC side down on an MEA with either 512 electrodes spaced 60 µm apart, or 519 electrodes with 30 µm spacing (Field et al., 2010 (link); Frechette et al., 2005 (link); Ravi et al., 2018 (link)). Oxygenated Ames perfused the retina throughout the experiment at a rate of 6–8 mL/min, heated to 32°C.

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Scalabrino M.L., Thapa M., Chew L.A., Zhang E., Xu J., Sampath A.P., Chen J, & Field G.D. (2022). Robust cone-mediated signaling persists late into rod photoreceptor degeneration. eLife, 11, e80271.

Publication 2022

Ames solution

Ames Darkness Dissection Eyes Light Mice Retina Vitrectomy

Corresponding Organization :

Other organizations : Duke University, Doheny Eye Institute, University of California, Los Angeles, University of Southern California

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Variable analysis

independent variables

Overnight dark adaptation of mice by placing their home cage in a light-shielded box

dependent variables

Retinal ganglion cell (RGC) activity measured using multi-electrode array (MEA)

control variables

Oxygenated Ames solution (Sigma, A1420) used to perfuse the retina throughout the experiment at a rate of 6–8 mL/min, heated to 32°C
All dissection procedures carried out in complete darkness using infrared converters and cameras

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