Chemical deglycosylation of rLdpA was performed using trifluoromethanesulfonic acid (TFMS) (Sigma-Aldrich), as described previously [35 (link),36 (link)]. After treatment with TFMS, the shift in protein Mr was assessed by SDS-PAGE. Bovine fetuin (Sigma-Aldrich) and BSA (Sigma-Aldrich) were used as glycoprotein and non-glycoprotein controls, respectively.
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