Characterization of mAb TS63 against CD63

The mAb TS63 directed to CD63 has been previously described [9 (link),21 (link)]. It was produced after immunizing BALB/c mice with a mixture of HEL and Jurkat cells. Spleen cells were fused with P3 × 63AG8 mouse myeloma cells according to standard techniques, distributed into 96-well tissue culture plates, and screened by indirect immunofluorescence and immunoprecipitation. Other antibodies were an anti-GM130 (clone 35, BD transduction, IgG1), an anti-EEA1 (clone 14, BD transduction, IgG1), an anti-PDI (RL90, IgG2a, Abcam), an anti-LAMP2 (H4B4, Santa Cruz, IgG1) and an anti-CD9 (TS9, IgG1, produced in our laboratory). The goat anti mouse IgG1, IgG2a and IgG2b antibodies, coupled to Alexa Fluor 488, Alexa Fluor 568 or Alexa Fluor 647 dyes, the goat anti-rabbit Ig coupled to Dylight 650 and the goat anti-mouse Ig coupled to Alexa Fluor 680 were obtained from Thermo Fisher Scientific.