Facial EMG Measurement Protocol

We recorded the EMG activity from the OO muscles of both sides using a pair of surface electrodes per side, with the active electrode on the lower eyelid and the reference electrode 3 cm away on the lateral canthus (Figure 1) [34 (link),35 (link)]. A square electrode (32 × 32 mm) was positioned on the forehead as a ground electrode. The EMG raw signals were amplified and bandpass-filtered (20 Hz–3 kHz) by a Digitimer D360 amplifier (Digitimer Ltd., Welwyn Garden City, Herts, UK), that digitized at a sampling rate of 5 kHz (CED 1401 laboratory interface; Cambridge Electronic Design, UK), and was stored on a laboratory computer for online visual display. The data were analyzed offline with dedicated software (Signal software; Cambridge Electronic Design) [16 (link),36 (link)].

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Paparella G., De Biase A., Cannavacciuolo A., Colella D., Passaretti M., Angelini L., Guerra A., Berardelli A, & Bologna M. (2022). Validating a Portable Device for Blinking Analyses through Laboratory Neurophysiological Techniques. Brain Sciences, 12(9), 1228.

Publication 2022

D360 amplifier CED 1401 laboratory interface Signal software

Eyelid Forehead Lateral canthus Muscles

Corresponding Organization : Sapienza University of Rome

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Variable analysis

independent variables

Position of active electrode on the lower eyelid
Position of reference electrode 3 cm away on the lateral canthus

dependent variables

EMG activity from the OO muscles of both sides

control variables

Square electrode (32 × 32 mm) positioned on the forehead as a ground electrode
EMG raw signals amplified and bandpass-filtered (20 Hz–3 kHz) by a Digitimer D360 amplifier
EMG signals digitized at a sampling rate of 5 kHz (CED 1401 laboratory interface)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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