Immunohistochemical Analysis of Zebrafish Hearts

IHC of embryonic zebrafish hearts for quantification of cardiomyocytes was performed as previously described^{38 (link)}. Primary antibodies used were: Amhc (Atrial myosin heavy chain; University of Iowa Developmental Studies Hybridoma Bank) and Living Colors® polyclonal anti-DsRed antibody (Clontech). Secondary antibodies used were anti-mouse IgG1-FITC (Southern Biotech) and anti-rabbit IgG-TRITC (Southern Biotech). A Zeiss M2BioV_12 Stereo microscope was used to image hearts. Additional antibody information is included in Supplementary Table 2.

Free full text: Click here

Gafranek J.T., D’Aniello E., Ravisankar P., Thakkar K., Vagnozzi R.J., Lim H.W., Salomonis N, & Waxman J.S. (2023). Sinus venosus adaptation models prolonged cardiovascular disease and reveals insights into evolutionary transitions of the vertebrate heart. Nature Communications, 14, 5509.

Publication 2023

Living Colors anti-DsRed antibody anti-mouse IgG1-FITC

Anti antibody Anti igg Antibodies Antibody Atrial Cardiomyocytes Embryonic Fitc Hearts Hybridoma Igg1 Microscope Mouse Myosin heavy chain Rabbit Tritc Zebrafish

Corresponding Organization :

Other organizations : University of Cincinnati Medical Center, Stazione Zoologica Anton Dohrn, Cincinnati Children's Hospital Medical Center, University of Colorado Anschutz Medical Campus

Top 5 similar protocols

Variable analysis

independent variables

Primary antibodies used: Amhc (Atrial myosin heavy chain; University of Iowa Developmental Studies Hybridoma Bank) and Living Colors® polyclonal anti-DsRed antibody (Clontech)

dependent variables

Quantification of cardiomyocytes in embryonic zebrafish hearts

control variables

Secondary antibodies used: anti-mouse IgG1-FITC (Southern Biotech) and anti-rabbit IgG-TRITC (Southern Biotech)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!