An inbred line of Chinese cabbage (B. rapa ssp. pekinensis cv. Bre) was used in this study. The seeds were sown in a greenhouse on August 8, 2014. Two weeks later, the seedlings were transplanted into the field at the Songjiang Farm Station of SIPPE in early September.
The in planta transformation procedures with Bre using the vernalization-infiltration method are described by Bai et al.22 . Briefly, Brassica plants with small flower buds at the early bolting stage were used for transformation. The plants were placed upside down in a vacuum desiccator that contained both infiltration media and the engineered Agrobacterium for vacuum infiltration. The Agrobacterium-infected plants were then grown in a dark room and incubated at 22/18 °C, after which they were transferred to a chamber room after 2 days. The pollen of the Bre plants was then used to pollinate the transformed flowers manually. The seeds of the transgenic plants were harvested after they plants grew for 1–2 months in a growth chamber.
Arabidopsis thaliana wild-type plants and hyl1 (Nossen ecotype) mutants were used in this study. The growth conditions and transgenic methods are described by Wu et al.19 (link).
The in planta transformation procedures with Bre using the vernalization-infiltration method are described by Bai et al.22 . Briefly, Brassica plants with small flower buds at the early bolting stage were used for transformation. The plants were placed upside down in a vacuum desiccator that contained both infiltration media and the engineered Agrobacterium for vacuum infiltration. The Agrobacterium-infected plants were then grown in a dark room and incubated at 22/18 °C, after which they were transferred to a chamber room after 2 days. The pollen of the Bre plants was then used to pollinate the transformed flowers manually. The seeds of the transgenic plants were harvested after they plants grew for 1–2 months in a growth chamber.
Arabidopsis thaliana wild-type plants and hyl1 (Nossen ecotype) mutants were used in this study. The growth conditions and transgenic methods are described by Wu et al.19 (link).
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