Investigating AMPK and PGC1α Inhibitors in Muscle Cell Differentiation

Dorsomorphin dihydrochloride (Compound C dihydrochloride, 10 μM, MedChemExpress, China) [27 (link)] was used to inhibit the activity of AMPK and SR-18292 (20 μM, MedChemExpress, China) [28 (link)] was used to inhibit the activity of PGC1α, respectively. After 5 days of differentiation, C2C12 cells were incubated with the intervention for 18 h in the CON group (PBS), DEX group (100 μM dexamethasone), DG group (100 μM dexamethasone + 5 × 10⁸ particles/mL GqDNVs), CC group (10 μM Compound C dihydrochloride + 100 μM dexamethasone + 5 × 10⁸ particles/mL GqDNVs) and SR group (20 μM SR-18292 + 100 μM dexamethasone + 5 × 10⁸ particles/mL GqDNVs).

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Zhou X., Xu S., Zhang Z., Tang M., Meng Z., Peng Z., Liao Y., Yang X., Nüssler A.K., Liu L, & Yang W. (2024). Gouqi-derived nanovesicles (GqDNVs) inhibited dexamethasone-induced muscle atrophy associating with AMPK/SIRT1/PGC1α signaling pathway. Journal of Nanobiotechnology, 22, 276.

Publication 2024

SR-18292

Corresponding Organization :

Other organizations : Huazhong University of Science and Technology, University of Tübingen

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Variable analysis

independent variables

Dorsomorphin dihydrochloride (Compound C dihydrochloride, 10 μM, MedChemExpress, China)
SR-18292 (20 μM, MedChemExpress, China)
Dexamethasone (100 μM)
GqDNVs (5 × 10^8 particles/mL)

dependent variables

Not explicitly mentioned

control variables

C2C12 cells

controls

Positive control: CON group (PBS)
Negative control: DEX group (100 μM dexamethasone)

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