A multicolor flow cytometry microglia cell panel developed in our lab was used to identify microglia and their immunophenotype (25 (link)–27 (link)). Our staining method identified the following microglial markers: CD11bc, CD45, CD32, CD163, CD200R, RT1b. Aliquots of microglia cells were moved to BD Trucount tubes (BD Biosciences) to determine absolute cell counts. Data for microglial cells was acquired by an LSR-II Flow Cytometer (Beckman Coulter). Traditional flow cytometry analysis was performed with FlowJo vr10.6.1 (FlowJo, LLC; Ashland, Oregon).
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Multicolor Flow Cytometry Microglia Profiling
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Corresponding Organization : The University of Texas Health Science Center at Houston
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Variable analysis
independent variables
- None explicitly mentioned
- Absolute microglial cell counts
- Microglial immunophenotype (expression of CD11bc, CD45, CD32, CD163, CD200R, RT1b)
- None explicitly mentioned
- Positive control: None mentioned
- Negative control: None mentioned
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