Culturing Human and Mouse Melanoma Cell Lines

Human melanoma cell lines LOXIMVI and WM793 were purchased from ATCC (Manassas, VA, USA). Mouse melanoma cells established from the transplantable tumor cell line established from a Braf+/LSL-V600E;Tyr::CreERT2+/o;p16INK4a/ mouse were obtained from Dr. Zelenay (The University of Manchester) (18 (link)). Cells were maintained in Dulbecco modified Eagle medium containing 10% fetal bovine serum in a 5% carbon dioxide atmosphere.

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Kim S.H., Jason R., Cho S., Ogata D., Milton D.R., Peng W., Menter D.G., Ekmekcioglu S, & Grimm E.A. (2018). The COX2 effector microsomal PGE2 synthase-1 is a regulator of immunosuppression in cutaneous melanoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(5), 1650-1663.

Publication 2018

LOXIMVI WM793

Atmosphere Braf Carbon 10 Carbon dioxide Cell lines Cells Eagle Fetal bovine serum Human Melanoma Mouse P16ink4a Tumor cell line

Corresponding Organization : The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Cell line (LOXIMVI, WM793, mouse melanoma cells)

dependent variables

Not explicitly mentioned

control variables

Culture medium (Dulbecco modified Eagle medium containing 10% fetal bovine serum)
Atmospheric conditions (5% carbon dioxide atmosphere)

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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