SLC5A11 SNP rs11074656 (C/T), DRD2 SNPs rs6276 and rs6277 were identified using Taqman PCR probes (Thermo Fisher), a two-step fast cycling real-time PCR machine (BioRad CFX96 Connect, Hercules, CA, USA), and SNP determination by CFX Maestro 2. SLC5A11 mRNA was quantified using real-time quantitative reverse transcriptase PCR (qRT-PCR), as previous described [77 (link)]. Primers: SLC5A11 sense 5′-GCCTCCACAGTTAGATCCCC-3′; SLC5A11 anti-sense 5′-CAGAACTAGCACCGCGATG-3′; actin sense 5′-AGAAAATCTGGCACCACACC-3′; Actin anti-sense 5′-CTCCTTAATGTCACGCACGA-3′.
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