Fetal bovine serum, penicilin-streptomycin, Lipofectamine 2000, goat anti-mouse antibody coupled to Alexa 594 and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Invitrogen (Carlsbad, CA, USA). Bovine seroalbumin, paraformaldehyde, phosphate buffered saline (PBS) and the protease inhibitors cocktail were from Sigma Chemical Co. (St. Louis, MO, USA). Mouse anti Rab4, Rab11 or anti-CAV-1 antibodies were purchased from BD Biosciences. LysoTracker Red was from Molecular Probes. Ang-(1-7) was from Bachem. The plasmid containing MAS1R-YFP cDNA was obtained as previously described.17 (link) shRNA ß-arrestin2 was from SantaCruz. All other chemicals were analytical grade reagents of the highest purity available.