Flow cytometry was performed as previously described8 (link). Fluorescence data were collected using a FACSCanto II system (BD Biosciences), a SA3800 cell analyzer (Sony Biotechnology, Tokyo, Japan), or a MA900 cell sorter (Sony Biotechnology) according to staining using an anti-CD271 antibody (ME20.4; BioLegend, San Diego, CA, USA). Data were analyzed using FlowJo software (v10, FlowJo LLC, Ashland, OR, USA) and the CytoExploreR package in R software21 .
Nakazato A., Mochizuki M., Shibuya-Takahashi R., Fujimori H., Fujii K., Saijoh S., Morita S., Yamazaki T., Imai T., Sato I., Satoh K., Yamaguchi K., Sugamura K., Yasuda J., Matsuura K., Shojaku H., Asada Y, & Tamai K. (2022). RELA is required for CD271 expression and stem-like characteristics in hypopharyngeal cancer. Scientific Reports, 12, 17751.
Other organizations :
Miyagi Prefectural Hospital Organization, Tohoku Medical and Pharmaceutical University Hospital, National Cancer Center Hospital East, University of Toyama
Fluorescence data collection using different flow cytometry instruments (FACSCanto II, SA3800 cell analyzer, MA900 cell sorter)
dependent variables
Fluorescence data
control variables
Staining using an anti-CD271 antibody (ME20.4; BioLegend, San Diego, CA, USA)
controls
No positive or negative controls were explicitly mentioned in the provided information.
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