SARS-CoV-2 IgG Detection by ELISA

IgGs related to SARS-CoV-2 against the RBD domain of S protein were detected in the serum collected from vaccinated and nonvaccinated participants using an ELISA Kit UDITEST-V2G (IPN, Mexico City, Mexico), according to the manufacturer’s instructions [13 (link)]. The optical density (OD) was measured at 450 nm in a Multiskan Ascent spectrophotometer (Thermo Scientific, Waltham, MA, USA). Each sample was run in duplicate, and the values were averaged.

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Islas-Vazquez L., Cruz-Aguilar M., Velazquez-Soto H., Jiménez-Corona A., Pérez-Tapia S.M, & Jimenez-Martinez M.C. (2022). Effector-Memory B-Lymphocytes and Follicular Helper T-Lymphocytes as Central Players in the Immune Response in Vaccinated and Nonvaccinated Populations against SARS-CoV-2. Vaccines, 10(10), 1761.

Publication 2022

Multiskan Ascent spectrophotometer

Elisa Optical Protein domain Sars cov 2 Serum

Corresponding Organization : Universidad Nacional Autónoma de México

Other organizations : Direccion General de Epidemiologia, Instituto Politécnico Nacional

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Variable analysis

independent variables

Vaccination status (vaccinated vs. nonvaccinated participants)

dependent variables

Optical density (OD) of IgGs related to SARS-CoV-2 against the RBD domain of S protein

control variables

Assay protocol (ELISA Kit UDITEST-V2G from IPN, Mexico City, Mexico)
Spectrophotometer (Multiskan Ascent, Thermo Scientific, Waltham, MA, USA)
Wavelength (450 nm)
Sample replication (each sample run in duplicate)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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