AFM analysis was performed as described previously [82 (link)]. Shortly, amyloid samples were prepared by applying a drop of 10 μL medium on freshly cleaved mica, V1 grade (NanoAndMore GmbH, Germany). After incubation for 10 min, the sample was rinsed with deionised water (Merck Millipore Inc., Burlington, MA, USA) and dried under a gentle stream of argon. A Multimode 8 Nanoscope atomic force microscope (AFM, Bruker, Billerica, MA, USA) was used to image the surfaces of the mica substrate and the deposited amyloid structures.
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