Quantifying Meristematic Characteristics in Lateral Roots

The lateral roots were fixed in formalin–acetic acid–methanol (FAA) according to the method of Livingston et al.^{62 (link)}. Blocks were sectioned with a Leica RM2016 rotary microtome at 5 μm. Fast green FCF and safranin O were used to stain the sections. The lignified or corkified cell wall and vessel element will be dyed red and other tissues will be dyed green. Images were captured with a Nikon Eclipse E100. Subsequently, the length of the meristematic zone and the number of meristematic cells were analyzed using ImageJ 1.53.

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Wang S., Feng Y., Lou Y., Niu J., Yin C., Zhao J., Du W, & Yue A. (2023). 3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis. Scientific Reports, 13, 3902.

Publication 2023

Eclipse E100

Acetic acid Cell wall Fast green fcf Formalin Meristematic Methanol Microtome Roots Safranin o Stain Tissues Vessel

Corresponding Organization :

Other organizations : Shanxi Agricultural University

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Variable analysis

independent variables

Fixation method (formalin–acetic acid–methanol (FAA) according to the method of Livingston et al.)

dependent variables

Length of the meristematic zone
Number of meristematic cells

control variables

Sectioning thickness (5 μm)
Staining (Fast green FCF and safranin O)

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