Comprehensive Antibody Panel for Septin Proteins

Antibodies used were as follows: Anti-Septin2 (Cat# 07–1813; Millipore for Western blot; and Cat# HPA018481; Sigma-Aldrich for IF); additional anti-Septin2 antibody used for immunofluorescence was a generous gift from Dr. Elias Spiliotis (Drexel University, Philadelphia, PA; Spiliotis et al., 2008 (link); Fig. 1 A), anti-Septin9 (Cat# sc-293291; Santa Cruz), anti-Septin6 (Cat# sc-514806; Santa Cruz for Western blot), anti-SEPT7 (Cat# ab175229; Abcam for Western blot), anti-Cortactin (Cat# 05–180; Millipore), anti-MMP14 (MT1-MMP; Cat# MAB3328; Millipore), anti-Tks5 (fish, G-7; Cat# sc-376211; Santa Cruz), anti-FAK (Cat# 610087; BD PharMingen), anti-GAPDH (Cat# AM4300; Ambion), anti-mCherry (Cat# 5993–100; BioVision), FITC-conjugated AffiniPure Donkey anti-Rabbit IgG (H+L; Cat# 711–095-152; Jackson Immunoresearch), Rhodamine Red-X AffiniPure Donkey Anti-Rabbit IgG (H+L; Cat# 711–295-152; Jackson Immunoresearch), Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H+L; Cat# 711–605-152; Jackson Immunoresearch), Goat Anti-Mouse IgG1 Secondary Antibody (Atto 488; Cat# LS-C209452; LSBio), Purified Rat Anti-Mouse TER-119 rat anti-mouse erythrocyte (Cat# 550565; BD PharMingen), and IHCPlus PECAM-1/CD31 Antibody Monoclonal (Cat# LS-B3446; LSBio).

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Collins K.B., Kang H., Matsche J., Klomp J.E., Rehman J., Malik A.B, & Karginov A.V. (2019). Septin2 mediates podosome maturation and endothelial cell invasion associated with angiogenesis. The Journal of Cell Biology, 219(2), e201903023.

Publication 2019

anti-Cortactin anti-FAK anti-GAPDH anti-mCherry Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H+L;

Alexa fluor 647 Anti antibody Anti igg Antibodies Antibody Cat 152 Cortactin Donkey Erythrocyte Fish Fitc Gapdh Goat Igg1 Immunofluorescence Mmp14 Mouse Pecam 1 Rabbit Rhodamine Western blot

Corresponding Organization :

Other organizations : University of Illinois at Chicago, Illinois College

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Variable analysis

independent variables

Anti-Septin2 antibody (Cat# 07–1813; Millipore)
Anti-Septin2 antibody (Cat# HPA018481; Sigma-Aldrich)
Anti-Septin2 antibody (a generous gift from Dr. Elias Spiliotis, Drexel University, Philadelphia, PA)
Anti-Septin9 antibody (Cat# sc-293291; Santa Cruz)
Anti-Septin6 antibody (Cat# sc-514806; Santa Cruz)
Anti-SEPT7 antibody (Cat# ab175229; Abcam)
Anti-Cortactin antibody (Cat# 05–180; Millipore)
Anti-MMP14 (MT1-MMP) antibody (Cat# MAB3328; Millipore)
Anti-Tks5 antibody (Cat# sc-376211; Santa Cruz)
Anti-FAK antibody (Cat# 610087; BD PharMingen)
Anti-GAPDH antibody (Cat# AM4300; Ambion)
Anti-mCherry antibody (Cat# 5993–100; BioVision)
FITC-conjugated AffiniPure Donkey anti-Rabbit IgG (H+L) (Cat# 711–095-152; Jackson Immunoresearch)
Rhodamine Red-X AffiniPure Donkey Anti-Rabbit IgG (H+L) (Cat# 711–295-152; Jackson Immunoresearch)
Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Cat# 711–605-152; Jackson Immunoresearch)
Goat Anti-Mouse IgG1 Secondary Antibody (Atto 488) (Cat# LS-C209452; LSBio)
Purified Rat Anti-Mouse TER-119 rat anti-mouse erythrocyte (Cat# 550565; BD PharMingen)
IHCPlus PECAM-1/CD31 Antibody Monoclonal (Cat# LS-B3446; LSBio)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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