1×106 BMDMs or BMDCs per ml were left untreated or stimulated with 1 μg/ml LPS-EB (tlrl-eblps, Invivogen), 50 μg/ml Curdlan (Invivogen), 25 μg/ml zymosan A (Sigma), 100 nM CpG (Invivogen), 5 MOI Bacillus Calmette-Guerin (M. bovis-BCG) (45 (link)), or 1 μg/ml Imiquimod (Invivogen) for 16 h. Cytokine concentrations in supernatants were analyzed with the Ready-Set-Go ELISA kits detecting mouse IL-1β, IL-2, IL-6, IL-10, IL-12p40, IL-12p70, IL-23p19, and TNFα (all eBioscience) according to the manufacturer's instructions and measured at 450 nm with a SpectraMax M5 ELISA reader (Molecular Devices).
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Cytokine Profiling of Myeloid Cells
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Other organizations : New York University
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Variable analysis
independent variables
- 1 μg/ml LPS-EB (tlrl-eblps, Invivogen)
- 50 μg/ml Curdlan (Invivogen)
- 25 μg/ml zymosan A (Sigma)
- 100 nM CpG (Invivogen)
- 5 MOI Bacillus Calmette-Guerin (M. bovis-BCG) (45 (link))
- 1 μg/ml Imiquimod (Invivogen)
- Cytokine concentrations in supernatants (mouse IL-1β, IL-2, IL-6, IL-10, IL-12p40, IL-12p70, IL-23p19, and TNFα)
- 1×10^6 BMDMs or BMDCs per ml
- Untreated cells (negative control)
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