Carotenoid and Chlorophyll Extraction

Carotenoid and chlorophyll of fresh leaves were extracted with 80% (v/v) acetone and then measured with a UV−visible spectrophotometer (Hitachi−U2000, Hitachi, Ltd., Tokyo, Japan) according to the previous method [56 (link)]. UV−Vis spectrophotometer with a mounted 126 integrating sphere (BioMate 3S, Thermo Fisher Scientific Inc., MA, USA) was used to determine leaf absorption as described previously [51 (link)].

Free full text: Click here

Chen L.X., Mao H.T., Lin S., Din A.M., Yin X.Y., Yuan M., Zhang Z.W., Yuan S., Zhang H.Y, & Chen Y.E. (2023). Different Photosynthetic Response to High Light in Four Triticeae Crops. International Journal of Molecular Sciences, 24(2), 1569.

Publication 2023

Hitachi−U2000 BioMate 3S

Acetone Carotenoid Chlorophyll Leaf

Corresponding Organization : Sichuan Agricultural University

Top 5 similar protocols

Variable analysis

independent variables

Carotenoid and chlorophyll of fresh leaves

dependent variables

Carotenoid and chlorophyll content measured with a UV−visible spectrophotometer
Leaf absorption measured with a UV−Vis spectrophotometer with a mounted integrating sphere

control variables

80% (v/v) acetone used for extraction
Hitachi−U2000 UV−visible spectrophotometer used for measurement
BioMate 3S UV−Vis spectrophotometer with a mounted integrating sphere used for leaf absorption measurement

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!