Imaging Botryllus Colony Vasculature

Live imaging of the vasculature of Botryllus colonies labeled with BSA-Alexa 594 (A13101; Thermo Fisher Scientific, Waltham, MA) was carried out using a motorized fluorescence stereomicroscope MZ16FA (Leica, Germany) as previously described (Braden et al., 2014 (link)). Fixed tissue for in situ hybridization was imaged on an Olympus FluoView 1000 Spectral Confocal (Tokyo, Japan). Lipophilic staining of the vasculature labeled with CellMask Green (A37608; Thermo Fisher Scientific). Time-lapse movies (n = 5) taken as z-stacks were acquired with a Zeiss LSM 880 with Airyscan capabilities.

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Rodriguez D., Braden B.P., Boyer S.W., Taketa D.A., Setar L., Calhoun C., Maio A.D., Langenbacher A., Valentine M.T, & De Tomaso A.W. (2017). In vivo manipulation of the extracellular matrix induces vascular regression in a basal chordate. Molecular Biology of the Cell, 28(14), 1883-1893.

Publication 2017

BSA-Alexa 594 MZ16FA FluoView 1000 Spectral Confocal CellMask Green LSM 880

Alexa 594 Fluorescence In situ hybridization Tissue

Corresponding Organization :

Other organizations : University of California, Santa Barbara

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Variable analysis

independent variables

Botryllus colonies labeled with BSA-Alexa 594
Lipophilic staining of the vasculature labeled with CellMask Green

dependent variables

Live imaging of the vasculature of Botryllus colonies
Fixed tissue for in situ hybridization
Time-lapse movies of the vasculature

control variables

Motorized fluorescence stereomicroscope MZ16FA (Leica, Germany)
Olympus FluoView 1000 Spectral Confocal (Tokyo, Japan)
Zeiss LSM 880 with Airyscan capabilities

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