Method A: In the silver reduction, the methodology described previously was followed [17 (link)]. Briefly, approximately 10 g of F. oxysporum biomass was taken in a conical flask containing 100 mL of distilled water. AgNO3 solution (10-3 M) was added to the erlenmeyer flask and the reaction was carried out in the dark. Periodically, aliquots of the reaction solution were removed and the absorptions were measured using a UV-Vis spectrophotometer (Agilent 8453 – diode array).
Method B: Another test was also carried out as following: approximately 10 g of F. oxysporum biomass was taken in a conical flask containing 100 mL of distilled water, kept for 72 h at 28°C and then the aqueous solution components were separated by filtration. To this solution, AgNO3 (10-3 M) was added and kept for several hours at 28°C.
The silver nanoparticles were characterized by scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) at a voltage of 20 kV (Jeol – JSM-6360LV) and previously coated with gold under vacuum.
Method B: Another test was also carried out as following: approximately 10 g of F. oxysporum biomass was taken in a conical flask containing 100 mL of distilled water, kept for 72 h at 28°C and then the aqueous solution components were separated by filtration. To this solution, AgNO3 (10-3 M) was added and kept for several hours at 28°C.
The silver nanoparticles were characterized by scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS) at a voltage of 20 kV (Jeol – JSM-6360LV) and previously coated with gold under vacuum.
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