Murine Melanoma and Breast Cancer Models

C57BL/6 mice, Pmel transgenic mice carrying TCR gene specific for the mouse homolog (Pmel-17) of human gp100 (28 (link)), parental FVB mice and FVBN202 transgenic mice were purchased from the Jackson Laboratory (Bar Harbor, ME). Melanoma cell line B16-gp100 (8 (link)), mammary tumor MMC line (4 ), and DC1.2 DC line were maintained in DMEM media supplemented with 10% heat-inactivated FBS (Life Technologies, Grand Island, NY), 2 mM L-glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin. All experimental procedures were conducted according to the protocols approved by the Virginia Commonwealth University Institutional Animal Care and Use Committee (Richmond, VA).

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Qian J., Yu X., Liu Z., Cai J., Manjili M.H., Yang H., Guo C, & Wang X.Y. (2023). SRA inhibition improves antitumor potency of antigen-targeted chaperone vaccine. Frontiers in Immunology, 14, 1118781.

Publication 2023

C57BL/6 mice Pmel transgenic mice parental FVB mice FBS

C57bl mice Cell line Glutamine Human Institutional animal care and use committee Mammary tumor Melanoma Mice Parental Penicillin Pmel Streptomycin Tcr gene Transgenic mice

Corresponding Organization : Hunter Holmes McGuire VA Medical Center

Other organizations : Missouri University of Science and Technology

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Variable analysis

independent variables

C57BL/6 mice
Pmel transgenic mice carrying TCR gene specific for the mouse homolog (Pmel-17) of human gp100
Parental FVB mice
FVBN202 transgenic mice

dependent variables

Measured outcomes

control variables

DMEM media supplemented with 10% heat-inactivated FBS (Life Technologies, Grand Island, NY), 2 mM L-glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin

controls

B16-gp100 (melanoma cell line)
MMC line (mammary tumor)
DC1.2 DC line

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