Aggregated RNA differential expression data and study protocols were shared through the NASA OSDR with accession number: OSD-53072 (link). Plasma cell-free RNA samples for RNA-seq analysis were derived from blood samples collected from 6 astronauts before, during, and after the Spaceflight on the ISS. Mean expression values were obtained from normalized read counts of 6 astronauts for each time point. Heatmaps were made for the 21 genes key genes on the normalized values per time point using R package pheatmap (v1.0.12).
Cope H., Elsborg J., Demharter S., Mcdonald J.T., Wernecke C., Parthasarathy H., Unadkat H., Chatrathi M., Claudio J., Reinsch S., Zwart S., Smith S., Heer M., Muratani M., Meydan C., Overbey E., Kim J., Park J., Schisler J., Mason C., Szewczyk N., Willis C., Salam A, & Beheshti A. (2023). More than a Feeling: Dermatological Changes Impacted by Spaceflight. Research Square.
Other organizations :
University of Nottingham, University of Oxford, Georgetown University, Technical University of Munich, University of California, Berkeley, Princeton University, Ames Research Center, University of Tsukuba, Murata (United States), University of Bonn, Johnson Space Center, University of Erfurt, Cornell University, University of North Carolina at Chapel Hill, Ohio University, University of Bradford, King's College London, Broad Institute
Mean expression values of 21 key genes from normalized read counts of 6 astronauts for each time point
control variables
Plasma cell-free RNA samples for RNA-seq analysis derived from blood samples collected from 6 astronauts
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