Impact of moderate and strong suppression of RAD51 on genomewide
expression was demonstrated as described previously [35 (link)]. Transduced cells
were harvested at day seven after selection, total RNA isolated, and expression
profile evaluated using Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA). Gene
arrays were scanned on an Affymetrix scanner to obtain expression values, which
were normalized by dChip Analyzer, the software which uses “Invariant
Set” normalization and “model-based” approaches to make
arrays comparable and calculate normalized expression values[36 (link), 37 (link)].
expression was demonstrated as described previously [35 (link)]. Transduced cells
were harvested at day seven after selection, total RNA isolated, and expression
profile evaluated using Gene 1.0 ST Arrays (Affymetrix, Santa Clara, CA). Gene
arrays were scanned on an Affymetrix scanner to obtain expression values, which
were normalized by dChip Analyzer, the software which uses “Invariant
Set” normalization and “model-based” approaches to make
arrays comparable and calculate normalized expression values[36 (link), 37 (link)].
